Enzymic phosphorylation of creatine by 1:3-diphosphoglyceric acid.
نویسندگان
چکیده
2. Comparisons with synthetic 3-hydroxyand 4-hydroxy-pipecolic acids indicated that the isolated imino acid may not be 4-hydroxypipecolic acid, but 3-hydroxypipecolic acid. Strictly identical behaviour of natural and synthetic 3-hydroxypipecolic acid could not be demonstrated since the two substances differed in stereoisomeric composition. 3. 3-Hydroxyand 4-hydroxy-pipecolic acids have been synthesized.
منابع مشابه
Nicotinamide adenine dinucleotide-specific glyceraldehyde 3-phosphate dehydrogenase from Pisum sativum. Effect of nicotinamide adenine dinucleotide and related compounds on the enzyme-catalyzed arsenolysis of 1,3-diphosphoglyceric acid.
NADf-specific glyceraldehyde 3-phosphate dehydrogenase (EC 1.2.1.12) from pea seeds is shown to catalyze the arsenolysis of 1 ,3-diphosphoglyceric acid. The reaction shows an absolute requirement for NAD+ or analogs which will replace NAD+ in the enzyme-catalyzed oxidative phosphorylation of glyceraldehyde 3-phosphate. NADH, glyceraldehyde j-phosphate, or NADf analogs which are inhibitory or in...
متن کاملPurification and Characterization of Phosphoglycerate Mutase from Methanol - grown Hyphomicrobium x and Pseudomonas
Phosphoglycerate mutase has been purified from methanol-grown Hyphomicrobium x and Pseudomonas AMI by acid precipitation, heat treatment, ammonium sulphate fractionation, Sephadex G-50 gel filtration and DEAE-cellulose column chromatography. The purification attained using the Hyphomicrobium x extract was 72-fold, and using the Pseudomonas AMI extract, 140-fold. The enzyme purity, as shown by a...
متن کاملAcetyl phosphate formation catalyzed by glyceraldehyde-3-phosphate dehydrogenase.
Glyceraldehyde-3-phosphate dehydrogenase catalyzes the oxidative phosphorylation of n-glyceraldehyde-3-phosphate to 1,3-diphosphoglyceric acid (1, 2). The enzyme also catalyzes the oxidation of n-glyceraldehyde, although the product of this reaction has not been characterized (1,2). In the present study it is shown that acetaldehyde (3), propionaldehyde, and butyraldehyde also act as substrates...
متن کاملEffect of Salts on Inhibition of Chicken Muscle Adenosine Monophosphate Deaminase by Phosphate Esters and Inorganic Phosphate EFFECT OF 5,5’-DITHIOBIS(2-NITROBENZOIC ACID) ON ENZYME ACTIVITY AND INHIBITION
Inhibition of purified AMP-deaminase from chicken breast muscle by phosphate esters and inorganic phosphate has been investigated. When tested at a concentration of 3 mM, in the presence of 0.15 M potassium chloride, the order of effectiveness as inhibitors of deaminase activity was: 2,3diphosphoglyceric acid, 3-phosphoglyceric acid, 2-phosphoglyceric acid, 2-phosphoenolpyruvate, fructose 1,6di...
متن کاملThe sensitivity of hemoglobin oxygen affinity to diphosphoglycerate and the characteristic pH of methemoglobin.
The sensitivity of the oxygen affinity of a hemoglobin to 2,3-diphosphoglyceric acid concentration has been defined as the change in log1/2O2 (deltalogp1/2O2) which results from saturating the hemoglobin with 2,3-diphosphoglyceric acid. The sensitivity varies from one hemoglobin species to another and is linearly rated to the difference in the logarithm of the binding constants of 2,3-diphospho...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Biochemical journal
دوره 70 4 شماره
صفحات -
تاریخ انتشار 1958